Single Nucleotide Polymorphism Genotyping Using Allele-Specific PCR and Fluorescence Melting Curves
نویسندگان
چکیده
منابع مشابه
Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves.
We present a PCR method for identification of single nucleotide polymorphisms (SNPs), using allele-specific primers designed for selective amplification of each allele. Matching the SNP at the 3' end of the forward or reverse primer, and additionally incorporating a 3' mismatch to prevent amplification of the incorrect allele, results in selectivity of the allele-specific primers. DNA melting a...
متن کاملFluorescent allele-specific PCR (FAS-PCR) improves the reliability of single nucleotide polymorphism screening.
The detection of single nucleotide polymorphisms (SNPs) is becoming increasingly important in both the evaluation of candidate gene polymorphisms and fine mapping studies (4,7). Many techniques exist for the detection of SNPs within random sequences, such as single-strand conformation polymorphism (SSCP) (5,6), heteroduplex analysis and direct sequencing, but few techniques specifically identif...
متن کاملRapid characterization of DNA oligomers and genotyping of single nucleotide polymorphism using nucleotide-specific mass tags
Using currently available MS-based methods, accurate mass measurements are essential for the characterization of DNA oligomers. However, there is a lack of specificity in mass peaks when the characterization of individual DNA species in a mass spectrum is dependent solely upon the mass-to-charge ratio (m/z). Here, we utilize nucleotide-specific tagging with stable isotopes to provide internal s...
متن کاملRapid detection of aneuploidy (trisomy 21) by allele quantification combined with melting curves analysis of single-nucleotide polymorphism loci.
BACKGROUND Molecular approaches for the detection of chromosomal abnormalities will allow the development of rapid, cost-effective screening strategies. We present here a molecular alternative for the detection of aneuploidies and, more specifically, trisomy 21. METHODS We used the quantitative value of melting curve analysis of heterozygous genetic loci to establish a relative allelic count....
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References 1. Matarese V, Stone RL, Waggoner DW, Bernlohr DA. Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins. Prog Lipid Res 1989;28:245–72. 2. Baier LJ, Sacchettini JC, Knowler WC, Eads J, Paolisso G, Tataranni PA, et al. An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increase...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03345dd03